densitometry analysis (tina) (Raytest GmbH)

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Raytest GmbH densitometry analysis (tina)
Densitometry Analysis (Tina), supplied by Raytest GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/densitometry+analysis+(tina)/densitometry+analysis++tina+/10__1194_slash_jlr__m700581___jlr200-123-75-78
Average 90 stars, based on 1 article reviews

densitometry analysis (tina) - by Bioz Stars, 2026-10

90/100 stars

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Article Title: Involvement of HVEM receptor in activation of nuclear factor kappaB by herpes simplex virus 1 glycoprotein D.
Article Snippet: .. Comparative analysis of the bands was performed by quantitative densitometry using the Tina software (version 2.10, Raytest, Straubenhardt, Germany). .. All the samples were solubilized at 4°C in lysis buffer (50 mM Tris-HCl, pH 7.4, 1 mM EDTA, 1 mM EGTA, pH 7.4, 1% Triton-X, NaCl 150 mM, 0.25% sodium deoxycholate, 1% Nonidet P-40 and, freshly added, 1 mM Na3VO4, 20 mM Na3F completed with protease inhibitor cocktails (Roche Applied Science) and centrifuged at 10 000 g. An amount of 50 mg of proteins was boiled in SDS sample buffer (50 mM Tris-HCl, pH 6.8, 10 mM dithiothreitol, 2% SDS, 0.1% bromphenol blue, 10% glycerol), and then subjected to electrophoresis on 10% denaturing polyacrylamide gel, transferred to nitrocellulose membrane.

Polymerase Chain Reaction:

Article Title: Biomarine extracts significantly protect from ultraviolet A-induced skin photoaging: an ex vivo study.
Article Snippet: We tested the activity of the marine nutraceutical CL-1222 added with a coenzyme Q10 (CoQ10)–lutein– selenium component (Celergen , Laboratoires-Dom, Switzerland) to protect human fibroblasts against ultraviolet A (UVA)-induced photoaging.. Cells obtained from 22to 39-year-old healthy donors were pretreated with CL-1222 before UV irradiation, as compared with same quantity of the CoQ10–lutein–selenium component.. As compared to untreated control, UVA-irradiated samples exhibited a significant increase of secreted matrix metalloproteinase-1 (MMP-1) ( p < 0.001) with over four-fold MMP-1 upregulation ( p < 0.001).

Article Title: Phosphatidylserine prevents UV-induced decrease of type I procollagen and increase of MMP-1 in dermal fibroblasts and human skin in vivo
Article Snippet: .. After predenaturation at 94jC for 3 min, each cycle of PCR amplification consisted of denaturation at 94jC for 30 s, annealing at 60jC for 30 s, and elongation at 72jC for 30 s; 21 cycles were performed for 36B4 and procollagen a1(I), 24 cycles for MMP-1 and IL-6, and 30 cycles for COX-2, after a final extension at 72jC for 5 min. PCR products were resolved on 2.0% agarose gels, followed by quantification with a densitometry analysis (TINA; Raytest Isotopenme gerate, Straubenhardt, Germany) of bands (relative density normalized to 36B4). ..

Amplification:

other:

Article Title: The autoreactivity of B cells in hereditary angioedema due to C1 inhibitor deficiency
Article Snippet: Briefly, isolated B cells were lysed and proteins were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to nitrocellulose extra blotting membrane (Sartorius AG, Göttingen, Germany).

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